Magnetic separate "turn-on" fluorescent biosensor for Bisphenol A based on magnetic oxidation graphene.

Bisphenol A (BPA) is commonly considered to cause a health hazard to wildlife and humans, acting as an exogenous estrogen. Herein, a magnetic separate "turn-on" fluorescent method for the detection of BPA was proposed based on fluorescence resonance energy transfer (FRET) between fluorescein-labeled BPA aptamer and magnetic oxidation graphene (MGO). At different concentrations of BPA, the fluorescence intensity of the sensing system was varied. The detection limit of 0.071ng/mL was obtained with the linear range of 0.2-10ng/mL. The biosensor exhibited excellent anti-interference ability and selectivity in actual water samples.