2017

Lanthanide-based peptide biosensor to monitor CDK4/cyclin D kinase activity.

Chemical communications (Cambridge, England) González-Vera JA, Bouzada D, Bouclier C, Eugenio Vázquez M, Morris MC
阅读原文 PDF DOI PubMed

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中文摘要

英文摘要

We describe a lanthanide biosensor that responds to CDK4 kinase activity in melanoma cell extracts through a significant and dose dependent increase in luminescence, thanks to sensitization of a DOTA[Tb3+] complex incorporated into a CDK4 substrate peptide by a unique tryptophan residue in an adjacent phosphoaminoacid binding moiety.

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