组成图示
示意图生成中
传感器类型
—
检测对象
—
检测原理
—
检测灵敏度
—
效应效果
—
传感器的构成
—
中文摘要
—
英文摘要
Stimulated emission depletion (STED) microscopy is a popular super resolution imaging technique. Not only synthetic dyes and fluorescent proteins can be utilized as STED fluorophores, but also genetically encoded biosensors. Fusing the biosensor with proteins of interest allows subdiffraction imaging of intracellular macromolecular architecture with simultaneous extraction of functional information about cellular activities. Here, we describe a protocol for live-cell STED microscopy of the HyPer2 biosensor fused to cytoskeletal filaments.