A simple sonochemical approach to fabricate a urea biosensor based on zinc phthalocyanine/graphene oxide/urease bioelectrode.

A novel zinc phthalocyanine/graphene oxide (ZnPh/GO) nanocomposite modified glassy carbon electrode (GCE) was prepared by using sonochemical approach and simple drop casting method. Urease (Urs) was used as the specific enzyme for urea detection and was physically immobilized onto the surface of ZnPh/GO nanocomposite. The fabricated ZnPh/GO/Urs matrix was successfully characterized by UV-vis-spectroscopy, FT-IR spectroscopy, scanning electron microscopy (SEM), raman spectrum, thermogravimetric analysis, cyclic voltammetric (CV) and amperometric techniques. The electrocatalytic performance of the ZnPh/GO/Urs electrode was investigated by urea biosensor. Our results demonstrate that the modified electrode has excellent electrocatalytic activity towards the sensing of urea in 0.1 M phosphate buffer solution (PBS, pH 7.2). The biosensor tolerated a wide linear concentration range for urea from 0.4 to 22 μM (R2 = 0.991), with a detection limit of 0.034 µM (S/N = 3). The ZnPh/GO/Urs bioectrode has several excellent properties, including a fast response time, high reproducibility and stability.