Highly Sensitive Novel Biosensor Based on an Immobilized lac Repressor.

Sensitive repressors: The conformational change that occurs in the lac repressor protein when it binds to an inducer molecule has been used to develop a biosensor that permits the detection of the corresponding operator sequence or specific inducer molecules. A capacitive signal transducer was used to translate the conformational change of the lac repressor protein, covalently immobilized on a gold electrode (see schematic representation, A=bound inducer molecule or DNA), into a measurable signal.