Emerging nano-biosensing with suspended MNP microbial extraction and EANP labeling.

Emerging nano-biosensing with suspended MNP microbial extraction and EANP labeling may ensure a secure microbe-free food supply, as rapid response detection of microbial contamination is of utmost importance. Many biosensor designs have been proposed over the past two decades, covering a broad range of binding ligands, signal amplification, and detection mechanisms. These designs may consist of self-contained test strips developed from the base up with complicated nanoparticle chemistry and intricate ligand immobilization. Other methods use multiple step-wise additions, many based upon ELISA 96-well plate technology with fluorescent detection. In addition, many biosensors use expensive antibody receptors or DNA ligands. But many of these proposed designs are impracticable for most applications or users, since they don't FIRST address the broad goals of any biosensor: Field operability, Inexpensive, with Real-time detection that is both Sensitive and Specific to target, while being as Trouble-free as possible. Described in this review are applications that utilize versatile magnetic nanoparticles (MNP) extraction, electrically active nanoparticles (EANP) labeling, and carbohydrate-based ligand chemistry. MNP provide rapid pathogen extraction from liquid samples. EANP labeling improves signal amplification and expands signaling options to include optical and electrical detection. Carbohydrate ligands are inexpensive, robust structures that are increasingly synthesized for higher selectivity. Used in conjunction with optical or electrical detection of gold nanoparticles (AuNP), carbohydrate-functionalized MNP-cell-AuNP nano-biosensing advances the goal of being the FIRST biosensor of choice in detecting microbial pathogens throughout our food supply chain.