Highly sensitive fluorescence biosensor for intracellular telomerase detection based on a single patchy gold/carbon nanosphere via the combination of nanoflare and hybridization chain reaction.

How to in situ detect intracellular telomerase activity with high sensitivity still faces many challenges. This paper constructs a new fluorescence biosensing platform for the sensitive detection of intracellular telomerase activity via the combination of nanoflare and hybridization chain reaction (HCR)-based signal amplification on a single patchy gold/carbon nanosphere (PG/CNS), which has two or more distinct parts and allows hybridized-DNA (HS-DNA/Primer-DNA/Flare-DNA) and H1/H2-DNA (a pair of cross complementary DNA hairpins) to bind onto their surfaces via Au-S bond and electrostatic interaction, respectively. In the presence of telomerase, Primer-DNA (telomerase primer) extends at its 3' end to produce a telomeric repeated sequence, resulting in the release of Flare-DNA followed by the recovery of the fluorescence. Subsequently, the released Flare-DNA further initiates cross hybridization of H1 and H2 DNA from mimic-HCR system to amplify the fluorescence signal. The in vivo confocal microscopy studies demonstrate that resulting sensor can enter into the cancer cells such as A549 cells, and lead to the increase in luminescence, which is stronger than the sensor without the HCR-based signal amplification system. A linear relationship between the fluorescence intensity and the amount of A549 cells is observed, and the limit of detection of the sensor reaches about 280 A549 cells.