Monitoring matrix metalloproteases based on the selective interaction between an Ir(iii) solvent complex and a histidine-rich polypeptide.

A luminescent biosensor has been developed for matrix metalloproteinase 9 (MMP-9) assays based on the selective interaction between an Ir(iii) solvent complex and a histidine-rich peptide, which avoids the complicated double labeling of substrate polypeptides commonly-used in FRET MMP detections and provides a promising strategy for MMP detection in clinical applications.