Porous carbon supported nanoceria derived from one step in situ pyrolysis of Jerusalem artichoke stalk for functionalization of solution-gated graphene transistors for real-time detection of lactic acid from cancer cell metabolism.

Effective detection of biomarkers for tumor cells has been the focus of attention. In this work, we have successfully fabricated a highly sensitive sensor based on solution-gated graphene transistors (SGGT) for detecting lactic acid content accumulated in tumor cells through their glycolysis metabolism. The sensing mechanism of the lactic acid sensor is attributed to electrochemical catalysis of H2O2 produced by the oxidation of lactic acid by lactate oxidase near the gate electrode. The key component of the sensor is the functionalization of porous carbon loaded with ceria nanoparticles derived from a novel one step in situ pyrolysis of pretreated Jerusalem artichoke stalk, which significantly improved the sensor sensitivity, i.e. a detection limit as low as 300 nM and linear range from 3 μM to 300 μM. The optimized lactic acid sensor has successfully applied to the detection of lactic acid in practical cell culture samples with high credibility. The SGGT-based lactic acid biosensor shows great potential for the application in tumor microenvironment due to its superior biocompatibility and accuracy.