Impedimetric detection of Fumonisin B1 and its biointeraction with fsDNA.

The electrochemical interaction of mycotoxin, Fumonisin B1 (FB1) with fish sperm double stranded DNA (fsDNA) was investigated by using electrochemical impedance spectroscopy (EIS) technique. The pencil graphite electrodes were used for impedimetric detection of fsDNA and FB1, and also the impedimetric monitoring of surface confined interaction of FB1 with fsDNA under the optimized conditions. The experimental conditions; the concentration of DNA and FB1 and the interaction time of FB1 with fsDNA were firstly optimized. The main features of impedimetric DNA biosensor, such as its detection limit with the reproducubility were also discussed. The selectivity of detection of interaction between FB1 and fsDNA was also explored in the mixture samples containing FB1 and each of other mycotoxins, such as zearalenone (ZEN), deoxyvinalenol (DON) in order to examine the selectivity of impedimetric DNA biosensor.