Dual amplification ratiometric biosensor based on a DNA tetrahedron nanostructure and hybridization chain reaction for the ultrasensitive detection of microRNA-133a.

A novel ratiometric electrochemiluminescence (ECL)-electrochemical (EC) hybrid biosensor with a high accuracy and reproducibility was fabricated for the ultrasensitive detection of miRNA-133a. With the help of a DNA tetrahedron nanostructure and hybridization chain reaction dual amplification strategy, the detection limit of 12.17 aM for miRNA-133a was obtained.