An ultrasensitive electrochemiluminescence biosensor for nuclear factor kappa B p50 based on the proximity hybridization-induced hybridization chain reaction.

Nuclear factor kappa B p50 (NF-κB p50) induces various biological processes. In this study, a highly selective and sensitive electrochemiluminescence (ECL) biosensor for the detection of NF-κB p50 has been developed, which combines the high selectivity of the proximity hybridization assay (PHA) with the high efficiency of the hybridization chain reaction (HCR).