Fluorescence biosensor based on silicon quantum dots and 5,5'-dithiobis-(2-nitrobenzoic acid) for thiols in living cells.

An efficient and stable fluorescent sensor is described for the detection and imaging of thiols. It is making use of silicon quantum dots (SiQDs) which can be rapidly prepared. They were characterized by transmission electron microscopy, X-ray power diffraction, Fourier transform infrared spectroscopy, X-ray photoelectron spectrometry. The SiQDs have an absorption maximum at 300 nm and displayed blue-green fluorescence with excitation/emission maxima at 410/480 nm. A mixture of SiQDs and 5,5'-dithiobis-(2-nitrobenzoic acid) (DTNB) exhibits strong fluorescence emission which however is quenched within 30 s of incubation with thiols. This is assumed to be due to an inner filter effect caused by the reaction of DTNB and thiols. The following thiols were tested: cysteine, homocysteine, and glutathione. The sensor has a linear response in the 3-100 μM thiol concentration range, and the LODs are between 0.80 and 0.96 μM. The sensor displays low cytotoxicity and was applied to fluorescence imaging of MCF-7 cells and Hela cells where it demonstrated excellent biocompatibility.