Design and fabrication of a label-free aptasensor for rapid and sensitive detection of endoglucanase.

Endoglucanase (endocellulase, EC 3.2.1.4) is one of the most widely used enzymes in industry. Despite its importance, improved methods for the rapid, selective, quantitative assay of this enzyme have been slow to emerge. In this work, we have designed an aptasensor platform for ultrasensitive endoglucanase II detection based on DNA aptamer and reduced graphene oxide/Au nanoparticles (RGO/AuNPs). The surface morphology of RGO/AuNPs was characterized by various techniques such as transmission electron microscopy (TEM), X-ray diffraction (XRD), energy-dispersive X-ray spectroscopy (EDX) methods. The aptasensor characterization was monitored with electrochemical techniques. Using RGO/AuNPs as a nanomaterial can effectively increase the conductivity of biosensor electrode. Moreover, using an RGO/AuNPs/aptamer platform in the presence of endoglucanase, the sensor system is able to generate a signal, which significantly improves the selectivity of the aptasensor. The fabricated aptasensor exhibits high sensitivity and selectivity to endoglucanase II with a low limit of detection (LOD) ˂0.1 nM.