The hot-wire concept: Towards a one-element thermal biosensor platform.

In this work, the 3ω hot-wire concept is explored as a prospective biosensing platform with a single sensing element that can detect analytes based on a change in the thermal interface conductance. A uniform receptor layer such as single-stranded DNA is immobilized on a thin aluminium wire, which serves not only as an immobilization platform but also as a heating element and temperature sensor together. The wire is heated periodically with an alternating current (angular frequency ω) and the third harmonic (frequency 3ω) of the voltage across the wire renders the efficiency of heat transfer from the wire to the surrounding medium. The amplitude of the 3ω voltage depends sensitively on the composition and conformation of the biofunctional interface layer. We illustrate this with a model system that includes blank aluminium wires, wires with silanes bound covalently to the native surface oxide, and with single-, respectively double-stranded DNA tethered to the silanes. The difference in heat-transfer due to these coatings is significant and measurable not only in a liquid but also in air. Based on this proof-of-concept, various applications come in sight such as mutation analysis and analyte detection with aptamers or molecularly-imprinted polymers as receptors. Wire materials other than aluminium are possible as well and the concept is suitable for miniaturization and parallelization.