Ultrasensitive biosensing platform based on yttria doped zirconia-reduced graphene oxide nanocomposite for detection of salivary oral cancer biomarker.

Herein, we report results of the studies relating to the fabrication of yttria-doped zirconia-reduced graphene oxide nanocomposite (nYZR) based biosensing platform for detection of salivary CYFRA-21-1 biomarker. The nYZR nanocomposite was hydrothermally synthesized and amine-functionalized using 3-aminopropyl triethoxysilane (APTES). This functionalized nanocomposite (APTES/nYZR) was electrophoretically deposited (45 V; 3 min) onto pre-hydrolyzed indium tin oxide (ITO) coated glass substrate (APTES/nYZR/ITO) followed by biofunctionalization via covalent immobilization of the anti-CYFRA-21-1 antibodies (anti-CYFRA-21-1/APTES/nYZR/ITO). The synthesized nanomaterial and the fabricated electrodes were characterized to investigate crystal structure, morphology and electrochemical properties via X-ray diffraction, Fourier transform infrared spectroscopy, scanning electron microscopy, transmission electron microscopy, cyclic voltammetry, differential pulse voltammetry and electrochemical impedance spectroscopy. The fabricated biosensing electrode (BSA/anti-CYFRA-21-1/APTES/nYZR/ITO) has an operating shelf life of 56 days and can be used to detect salivary CYFRA-21-1 biomarker concentration as low as 7.2 pg mL-1 with wide linear detection range of 0.01-50 ng mL-1. This work opens new opportunities to explore the electrochemical behavior of nanostructured yttria stabilized zirconia (YSZ) and its composites at room temperature and its utility in developing biosensors and other electrochemical devices.