Recent advances and perspectives of enzyme-based optical biosensing for organophosphorus pesticides detection.

The overuse or abuse of organophosphorus pesticides (OPs) can bring about severe contamination problems in foodstuff and the environment, which will seriously threaten human health and the ecosystem's cycle. Hence, it is in high demand to establish sensitive, portable, specific, and cost-effective methods for monitoring OPs to control food safety, protect the ecosystem, and prevent disease. The optical biosensor with enzyme as bio-recognition elements has been an effective alternative for OPs detection. Herein, we firstly introduce various enzymes, sensing mechanisms, advantages and disadvantages used as bio-recognition elements in optical sensing for OPs detection. Then, we review various optical biosensing strategies based on enzymes as recognition elements that were ingeniously designed and successfully utilized for OPs detection, with a particular emphasis on photoluminescence (PL), chemiluminescence (CL), electrochemiluminescence (ECL), and colorimetric (CM) biosensing strategies. We not only highlight the state-of-art developments and the construction strategies of the enzyme-based optical biosensing method but also summarize the existing deficiencies, current challenges, and the future perspectives of OPs detection.