Facile Fabrication of Highly Porous Bioelectrode Membrane by Combining Breath Figure Process and Self-Assembly Process with Amphiphilic Diblock Copolymer and Hydrophilic Biocatalyst.

This study demonstrated a facile method to fabricate a bioelectrode immobilizing a hydrophilic biocatalyst via self-assembling of the biocatalyst with an amphiphilic diblock copolymer (di-BCP) in the breath figure process. Poly(methyl methacrylate)-block-poly(acrylic acid) (PMMA-b-PAA) as an amphiphilic di-BCP and glucose oxidase (GOD) as a hydrophilic biocatalyst were used in this model study. The electrode membrane presented a highly porous morphology, bringing a large surface area and high permeability to reactants. The sensing performances of the bioelectrode were tested to rapidly and conveniently detect glucose. The results showed that PMMA-b-PAA and GOD had good synergism on the electrocatalytic activity of GOD, and its current response to glucose was remarkably enhanced. The fabricated electrode presented a good reproducibility and durability. The blood glucose test provided a value well consistent with the one measured by an automatic biochemical analyzer with an enzymatic method from a hospital. This technique would be anticipated to build a platform to rapidly and conveniently construct a highly sensitive biosensor immobilizing a hydrophilic biocatalyst.