Involvement of O

Relationship between mycotoxin-induced hormesis and reactive oxygen species (ROS) has not been systematically investigated due to the lack of an effective analysis method. To monitor cellular release and intracellular level of O2·-, carboxymethyl cellulose-Mn3(PO4)2 nanocomposite was synthesized to fabricate an electrochemical biosensor, which selectively detects O2·- over the range of 57.50 nM ∼ 2.95 μM (R2 = 0.99) with the sensitivity of 78.67 μA μM-1 cm-2 and the detection limit of 8.47 nM. Transient exposure to zearalenone (ZEA) induces the enhancement on cell viability, immediate O2·- release from cells, and reduction of intracellular O2·- level. After post-treatment culture, intracellular O2·- initially increases to a high level and then decreases to the normal level. Concurrently, the ZEA-induced hormesis disappears. Based on the findings, we propose a mechanism, involving the ROS release, increase of succinate dehydrogenase activity and recovery of intracellular ROS, to explain the occurrence and disappearance of hormesis in intestinal porcine enterocytes.