Quantum Dots for Monitoring Choline Consumption Process of Living Cells via an Electrostatic Force-Mediated Energy Transfer.

In this work, a ratiometric nanoprobe CdS/ZnS-FB was designed for H2O2 detection based on FRET assay. Furthermore, CdS/ZnS-FB could work for detecting choline (Ch) and acetylcholine (ACh) since H2O2 is the enzyme cascade reaction product. Significantly, the Jurkat T's choline consumption could also be quantitatively measured by monitoring FRET ratio (I522/I426). Thus, the biosensor could be applied as a universal tool for the detection of choline consumption of living cells, which provides a good potential for the applications in detecting chemical transmitter and cancer diagnosis.