Studies of interactions between fibronectin and a specific antibody against fibronectin using SPRi and QCM.

Surface Plasmon Resonance Imaging (SPRi) and the Quartz Crystal Microbalance (QCM) technique were used to characterize the interactions between fibronectin and a specific monoclonal antibody against fibronectin. These techniques were used to investigate the formation of successive layers of the biosensor and how they affect the biosensor's response. The analytical response of both detectors to fibronectin concentration is linear in the range 5-100 ng/mL. The changes in mass on the surface of a quartz crystal covered with a layer of gold during biosensor formation were investigated with a QCM. The equilibrium constant was determined to be KC = 1.22∙108 dm3/mol and the dissociation constant to be KD= 8.20 ∙ 10-9 mol/dm3.