Cytochrome c-multiwalled carbon nanotube and cobalt metal organic framework/gold nanoparticle immobilized electrochemical biosensor for nitrite detection.

Based on cytochrome c-multiwalled carbon nanotubes (Cyt c-MWCNTs) and cobalt metal-organic frameworks/gold nanoparticles (Co-MOFs/AuNPs), an electrochemical biosensor was proposed for the detection of nitrite. Herein, Co-MOFs and AuNPs were immobilized on gold electrodes via surface layer assembly. Their advantages including large surface area and high conductivity provided an excellent platform for the immobilization of Cyt c-MWCNTs. Cyt c-MWCNTs were prepared via electrostatic adsorption and possessed good biocompatibility and superior electrocatalytic activity towards nitrite. Notably, MWCNTs and AuNPs could provide a good microenvironment for the electron transfer of Cyt c, which further significantly promoted the dispersion of MWCNTs. All of the above features led to outstanding electrochemical performance and achieved signal amplification for nitrite detection. Therefore, the biosensor displayed a linear range from 0.005 μmol L-1 to 1000 μmol L-1 with a detection limit of 0.0044 μmol L-1 for nitrite detection. In addition, the designed biosensor exhibited excellent selectivity and could be applied in real samples.