Design, optimization and application of a highly sensitive microbial electrolytic cell-based BOD biosensor.

Biochemical oxygen demand (BOD) is an important biochemical indicator for determining the degree of water pollution and guiding the design of wastewater treatment processes. BOD sensors based on microbial electrochemical technology can conduct real-time online monitoring of organic matter and have attracted extensive attention. However, research on microbial electrolytic cell (MEC)-type BOD sensors is at the stage of theoretical exploration. Here, we designed and optimized a highly sensitive MEC-type BOD sensor by screening inoculants, comparing electrode materials, and optimizing the reactor configuration. The results showed that effective means to optimize a BOD sensor for fast activation and sensitive testing included the inoculation of the MEC reactor effluent with large amounts of biomass and highly active bacteria, selection of carbon felt electrodes with excellent adsorption and permeability, miniaturization of the reactor, regulation of suitable electrode spacing, and design of the penetrating fluid structure. Then, the optimized sensing system was applied to determine the BOD concentration in model solutions of sodium acetate in a laboratory environment, where it accurately measured BOD concentrations in the range of 10-500 mg/L and maintained good parallelism during long-term operation. Next, the MEC-type BOD sensors were put into practice in the field as an alarm for accidents at an actual sewage plant. The whole BOD sensing system was quickly assembled on site and started up, and it gave an early warning shortly after the concentration of organic matter in the water suddenly increased, thus showing a high potential for engineering applications. This study broadened the domains of application of MEC-type BOD sensors in environmental monitoring, and promoted the development of technological innovation in water ecology and environmental monitoring.