Recombinant protein G/Au nanoparticles/graphene oxide modified electrodes used as an electrochemical biosensor for

UNLABELLED: In this study, a simple label-free biosensor for Brucella was constructed, which based on the screen-printed carbon electrode (SPCE) modified by Recombinant protein G/gold nanoparticles/graphene oxide (RpG/Au/GO). The impedance responses of the proposed biosensor were measured by electrochemical AC impedance method in Brucella antigen gradient concentration solutions. The results showed that the linear range of this biosensor was from 1.6 × 102 CFU/mL to 1.6 × 108 CFU/mL with the minimum detection limit of 3.2 × 102 CFU/mL (S/N = 3). Moreover, the biosensor for Brucella detection possessed acceptable reproducibility with a relative standard deviation of 5.15% and acceptable stability with a relative standard deviation of 4.68%. The spiked recovery rate in actual pasteurized milk samples was more than 92%. Therefore, the developed biosensor exhibits excellent prospects in the selective quantification detection of Brucella abortus. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s13197-022-05544-8.