Integration of enzyme-encapsulated mesoporous silica between nanohole array electrode and hydrogel film for flow-type electrochemical biosensor.

We herein propose a simple and sensitive electrochemical flow biosensor platform without an external flow device. The sensing unit comprises a platinum nanohole array electrode deposited on a nanoporous track-etched membrane (PtNH/NPM), a packed-layer of glucose oxidase-encapsulated mesoporous silica particles (GOD/MPS), and bovine serum albumin hydrogel film (BSA gel film). This sensing unit was fixed at the open window at the side of the plastic container with internal solution containing NaCl as osmotic reagent. When the sample glucose solution (0.10 mL) was pipetted at the sensing unit, a portion of the sample solution (5 μL) was spontaneously transferred into the BSA gel film. The concentration gradient of NaCl between the internal solution and the BSA gel film induced osmotic flow of water toward the internal solution. This osmotic flow assisted delivery of glucose to the GOD/MPS and enzymatically generated H2O2 to the PtNH/NPM. The proposed sensor could be used repeatedly and produced a linear current response for glucose, with a limit of detection of 16 μM. These sensor performances confirmed availability of the sensor design utilizing the osmotic flow.