Dendritic Gold Nanoparticles Loaded on 3D Graphene-like Surface and Layer-by-Layer Assembly for Enhanced Glucose Biosensing.

BACKGROUND/OBJECTIVES: In this study, AuDNs/EPLE composite electrodes with hierarchical dendritic nanogold structures were fabricated using the in situ electrodeposition of gold nanoparticles through the i-t method. METHODS: A conductive polymer composite membrane, PEDOT, was synthesized via the electropolymerization of EDOT and the negatively charged PSS-. The negatively charged SO3- groups on the surface of the PEDOT membrane were electrostatically adsorbed with the glucose oxidase (GOD) enzyme and a positively charged chitosan co-solution (GOD/chit+). Using a layer-by-layer self-assembly approach, GOD was incorporated into the multilayers of the composite electrode to create the composite GOD/chit+/PEDOT/AuDNs/EPLE. RESULTS: Electrochemical analysis revealed a GOD surface coverage of 8.5 × 10-10 mol cm-2 and an electron transfer rate of 1.394 ± 0.02 s-1. The composite electrode exhibited a linear response to glucose in the concentration range of 6.923 × 10-2 mM to 1.54 mM, with an apparent Michaelis constant of 0.352 ± 0.02 mM. Furthermore, the GOD/chit+/PEDOT/AuDNs/EPLE also showed good accuracy of glucose determination in human serum samples. CONCLUSIONS: These findings highlight the potential of the GOD/chit+/PEDOT/AuDNs/EPLE composite electrode in the development of efficient enzymatic biofuel cells for glucose sensing and energy harvesting applications.