Recent advances in glucose monitoring utilizing oxidase electrochemical biosensors integrating carbon-based nanomaterials and smart enzyme design.

Glucose oxidase (GOx), as a molecular recognition element of glucose biosensors, has high sensitivity and selectivity advantages. As a type of biosensor, the glucose oxidase electrode exhibits advantages such as ease of operation, high sensitivity, and strong specificity, promising broad application prospects in biomedical science, the food industry, and other fields. In recent years, with the advancement of nanotechnology, research efforts to enhance the performance of GOx biosensors have primarily focused on improving the conductive properties and specific surface area of nanomaterials, while neglecting the potential to modify the structure of the core component, GOx itself, to improve biosensor performance. Rapid modification of the GOx surface through chemical modification techniques yields a new modified enzyme (mGOx). Meanwhile, composite techniques involving carbon nanomaterials can be employed to further enhance sensor performance. This article reviews the construction methods and optimization strategies of glucose oxidase electrodes in recent years, along with research progress in their application in electrochemical sensing for glucose detection, and provides an outlook for future developments.