Optical Fiber TFBG Glucose Biosensor via pH-Sensitive Polyelectrolyte Membrane.

A novel glucose biosensor is developed based on a tilted fiber Bragg grating (TFBG) functionalized with a pH-responsive polyelectrolyte multilayer membrane, onto which glucose oxidase (GOD) is immobilized. The sensing film is constructed via layer-by-layer self-assembly of poly(ethylenimine) (PEI) and poly(acrylic acid) (PAA), which undergoes reversible swelling and refractive index (RI) changes in response to local pH variations. These changes are transduced into measurable shifts in the resonance wavelengths of TFBG cladding modes. The catalytic action of GOD oxidizes glucose to gluconic acid, thereby modulating the interfacial pH and actuating the polyelectrolyte membrane. With an optimized (PEI/PAA)4(PEI/GOD)1 structure, the biosensor achieves highly sensitive glucose detection, featuring a wide measurement range (10-8 to 10-2 M), a low detection limit of 27.7 nM, and a fast response time of ~60 s. It also demonstrates excellent specificity and robust performance in complex biological matrices such as rabbit serum and artificial urine, with recovery rates of 93-102%, highlighting its strong potential for point-of-care testing applications. This platform offers significant advantages in stability, temperature insensitivity, and miniaturization, making it well-suited for clinical glucose monitoring and disease management.