Composite transducers for amperometric biosensors. The glucose sensor.

A new concept of a composite transducer for amperometric biosensors based on the use of a solid substance with amphiphilic character (called a solid binding matrix, SBM) is presented. The electrochemical properties of the transducers prepared with five different SBMs and the characteristics and performance of SBM-based glucose sensors prepared by three different methods are described. Biosensor stability is evaluated and discussed. The biosensor was used for the determination of glucose in wine, yielding results which were consistent with those obtained with the commercially available Glucose Enzyme Photometric Kit. The average accuracy was 6% for the whole range of analyzed concentrations (0.2-47 g/L) using the same sample dilution in a buffer.