Disposable biosensor based on cathodic electrochemiluminescence of tris(2,2-bipyridine)ruthenium(II) for uric acid determination.

A new method for uric acid (UA) determination based on the quenching of the cathodic ECL of the tris(2,2-bipyridine)ruthenium(II)-uricase system is described. The biosensor is based on a double-layer design containing first tris(2,2-bipyridine)ruthenium(II) (Ru(bpy)3(2+)) electrochemically immobilized on graphite screen-printed cells and uricase in chitosan as a second layer. The uric acid biosensing is based on the ECL quenching produced by uric acid over the cathodic ECL caused by immobilized Ru(bpy)3(2+) in the presence of uricase. The use of a -1.1 V pulse for 1s with a dwelling time of 10s makes it possible to estimate the initial enzymatic rate, which is used as the analytical signal. The Stern-Volmer type calibration function shows a dynamic range from 1.0×10(-5) to 1.0×10(-3)M with a limit of detection of 3.1×10(-6)M and an accuracy of 13.6% (1.0×10(-4)M, n=5) as relative standard deviation. Satisfactory results were obtained for urine samples, creating an affordable alternative for uric acid determination.