Proteolysis-mediated protection of gold nanoparticles for sensitive activity assay of peptidases.

Rapid, sensitive and quantitative assays for peptide hydrolysis enzymes are of paramount importance for drug development and in the diagnosis of disease. Here, we proposed a novel biosensor for sensitive and selective active screening of peptidases. This strategy relies on the proteolysis-mediated protection of gold nanoparticles (AuNPs) that were decorated with biotin-labeled substrate peptides and can be aggregated by streptavidin. Enzyme-mediated protection of AuNPs offers this strategy high specificity, and the use of AuNPs additionally allows a visual and homogeneous assay format, thus permitting improved simplicity and throughput of the assays. As a model case, desirable selectivity and sensitivity in peptidase assay were achieved in the active assays of pancreatic elastase with a wide linear response range from 0.005 to 0.10 U/mL and a detection limit of 0.003 U/mL. The results indicated that this strategy can offer a simple, robust and convenient platform for visualized peptidase activity analysis and related biochemical studies with high sensitivity and selectivity.