Miniaturized integrated biosensors.

Miniaturized integrated thin-film biosensors were developed for use in clinical analyzers and for in vivo applications. A glucose and a lactate sensor were integrated with a pH-sensor on a flexible substrate. Both enzyme sensors are based on the electrochemical measurement of H2O2 produced by the enzymes glucoseoxidase and lactateoxidase respectively. The solid state pH-sensor uses a neutral carrier membrane. The intended application of this device is the monitoring of metabolic parameters in the intensive care unit and the operation theater and the use as a sensor module in clinical analyzers. The glucose-, lactate- and pH-sensor was tested in buffer solutions and undiluted serum showing excellent performance.