A regenerative electrochemical biosensor for mercury(II) by using the insertion approach and dual-hairpin-based amplification.

A simple and effective biosensor for Hg(2+) determination was investigated. The novel biosensor was prepared by the insertion approach that the moiety-labeled DNA inserted into a loosely packed cyclic-dithiothreitol (DTT) monolayer, improving the hybridization efficiency. Electrochemical impedance spectroscopy studies of two biosensors (single-hairpin and dual-hairpin structure DNA modified electrodes) used for Hg(2+) detection indicated that the dual-hairpin modified electrode had a larger electron transfer resistance change (ΔRct). Consequently, the dual-hairpin structure was used as a signal amplifier for the preparation of a selective Hg(2+) biosensor. This biosensor exhibited an excellent selectivity toward Hg(2+) over Cd(2+), Pd(2+), Co(2+) etc. Also, a linear relation was observed between the ΔRct and Hg(2+) concentrations in a range from 0.1 nM to 5 μM with a detection limit of 28 pM under optimum conditions. Moreover, the biosensor can be reused by using L-cysteine and successfully applied for detecting Hg(2+) in real samples.