Hierarchically mesostructured porous TiO

Effective immobilization of enzymes on an electrode surface is of great importance for biosensor development, but it still remains challenging because enzymes tend to denaturation and/or form close-packed structures. In this work, a free-standing TiO2 hollow nanofibers (HNF-TiO2) was successfully prepared by a simple and scalable electrospun nanofiber film template-assisted sol-gel method, and was further explored for glucose oxidase (GOD) immobilization and biosensing. This porous and nanotubular HNF-TiO2 provides a well-defined hierarchical nanostructure for GOD loading, and the fine TiO2 nanocrystals facilitate direct electron transfer from GOD to the electrode, also the strong interaction between GOD and HNF-TiO2 greatly enhances the stability of the biosensor. The as-prepared glucose biosensors show good sensing performances both in O2-free and O2-containing conditions with good sensitivity, satisfactory selectivity, long-term stability and sound reliability. The novel textile formation, porous and hierarchically mesostructured nature of HNF-TiO2 with excellent analytical performances make it a superior platform for the construction of high-performance glucose biosensors.