A microfluidic biosensor for the diagnosis of chronic wasting disease.
Cervids are affected by a neurologic disease that is always fatal to individuals and has population effects. This disease is called chronic wasting disease (CWD) and i...
Development of a workflow for the selection, identification and optimization of lactic acid bacteria with high γ-aminobutyric acid production.
Lactic acid bacteria produce γ-aminobutyric acid (GABA) as an acid stress response. GABA is a neurotransmitter that may improve sleep and resilience to mental stress. ...
Biosensors, Recent Advances in Determination of BDNF and NfL.
Key biomarkers such as Brain Derived Neurotrophic Factor (BDNF) and Neurofilament light chain (NfL) play important roles in the development and progression of many neu...
A three-colour stress biosensor reveals multimodal response in single cells and spatiotemporal dynamics of biofilms.
The plethora of stress factors that can damage microbial cells has evolved sophisticated stress response mechanisms. While existing bioreporters can monitor individual...
Real-time bio-layer interferometry ubiquitination assays as alternatives to western blotting.
Ubiquitination is a crucial cellular pathway enabling normal cellular functions. Abnormalities in the ubiquitination process can lead to cellular dysfunction and cause...
Diagnostic techniques for critical respiratory infections: Update on current methods.
Respiratory infections, whether chronic or acute, are frequent in both children and adults and result in an economic burden in health care systems. In particular, for ...
Chromophore carbonyl twisting in fluorescent biosensors encodes direct readout of protein conformations with multicolor switching.
Fluorescent labeling of proteins is a powerful tool for probing structure-function relationships with many biosensing applications. Structure-based rules for systemati...
A novel inductively coupled capacitor wireless sensor system for rapid antibiotic susceptibility testing.
BACKGROUND: The increasing prevalence and severity of antimicrobial resistance (AMR) present a major challenge to our healthcare system. Rapid detection of AMR is esse...
Identification and quantitative assessment of motor complications in Parkinson's disease using the Parkinson's KinetiGraph™.
INTRODUCTION: Effective management and therapies for the motor complications of Parkinson's disease (PD) require appropriate clinical evaluation. The Parkinson's Kinet...
A capacitive laser-induced graphene based aptasensor for SARS-CoV-2 detection in human saliva.
SARS-CoV-2 virus induced CoVID-19 pandemic has accelerated the development of diagnostic tools. Devices integrated with electrochemical biosensors may be an interestin...
Protocol for constructing GQD-PMO functionalized FET-biosensor for ultrasensitive exosomal miRNA detection.
Here, we present a protocol for constructing an ultrasensitive biosensor for exosomal-miRNA detection. We describe steps for preparing graphene quantum dot-phosphorodi...
Electrochemical Methods in the Cloud: FreiStat, an IoT-Enabled Embedded Potentiostat.
Embedded potentiostats enable electrochemical measurements in the Internet-of-Things (IoT) or other decentralized applications, such as remote environmental monitoring...
Algorithmic Programming of Sequential Logic and Genetic Circuits for Recording Biochemical Concentration in a Probiotic Bacterium.
Through the implementation of designable genetic circuits, engineered probiotic microorganisms could be used as noninvasive diagnostic tools for the gastrointestinal t...
Molding a PI(3,5)P2 biosensor.
The lipid phosphatidylinositol 3,5-bisphosphate-PI(3,5)P2-is known to be a key regulator of cellular traffic in health and disease, but its cellular localization was s...
Pb(II)-inducible proviolacein biosynthesis enables a dual-color biosensor toward environmental lead.
With the rapid development of synthetic biology, various whole-cell biosensors have been designed as valuable biological devices for the selective and sensitive detect...
Protegrin-2, a potential inhibitor for targeting SARS-CoV-2 main protease M
BACKGROUND: SARS-CoV-2 variants continue to spread throughout the world and cause waves of COVID-19 infections. It is important to find effective antiviral drugs to co...
Using Fiber Photometry in Mice to Estimate Fluorescent Biosensor Levels During Sleep.
Sleep is not homogenous but contains a highly diverse microstructural composition influenced by neuromodulators. Prior methods used to measure neuromodulator levels in...
Establishment of Human PD-1/PD-L1 Blockade Assay Based on Surface Plasmon Resonance (SPR) Biosensor.
Blockade of the programmed cell death protein 1 (PD-1)/PD-ligand 1 (PD-L1) axis is a promising strategy for cancer immunotherapy. Although antibody-based PD-1/PD-L1 in...
Biosensor Guided Polyketide Synthases Engineering for Optimization of Domain Exchange Boundaries.
Type I modular polyketide synthases (PKSs) are multi-domain enzymes functioning like assembly lines. Many engineering attempts have been made for the last three decade...
High-Throughput Effect-Directed Monitoring Platform for Specific Toxicity Quantification of Unknown Waters: Lead-Caused Cell Damage as a Model Using a DNA Hybrid Chain-Reaction-Induced AuNPs@aptamer Self-Assembly Assay.
A high-throughput cell-based monitoring platform was fabricated to rapidly measure the specific toxicity of unknown waters, based on AuNPs@aptamer fluorescence bioassa...