A ratiometric fluorescent biosensor for the sensitive determination of α-glucosidase activity and acarbose based on N-doped carbon dots.
本研究基于氮掺杂碳点(N-CDs)构建了一种用于α-葡萄糖苷酶(α-glu)及其抑制剂阿卡波糖(acarbose)检测的比率荧光传感平台。α-glu可催化α-熊果苷(α-arbutin)水解释放对苯二酚(HQ),生成的HQ在碱性条件下被氧化并与聚乙烯亚胺(PEI)共聚,形成具有510 nm黄绿色荧光的PHQ-PEI共聚物。PHQ-P...