Ultrasensitive photoelectrochemical biosensor for the detection of HTLV-I DNA: A cascade signal amplification strategy integrating λ-exonuclease aided target recycling with hybridization chain reaction and enzyme catalysis.
DNA 的灵敏特异检测对临床诊断具有重要意义。本文提出一种级联信号放大策略,并将其引入光电化学(PEC)生物传感器,用于超灵敏检测人 T 细胞淋巴病毒 I 型(HTLV-I)DNA。该策略整合 λ-外切酶(λ-Exo)辅助靶标循环、杂交链式反应(HCR)和酶催化。当存在 HTLV-I 靶 DNA(tDNA)时,设计的发夹 DNA(h...