Label-free DNAzyme for highly sensitive detection of multiple biomolecules in real samples through target-triggered catalytic cleavage reactions with auramine O's discriminated fluorescence emission.

本文基于可循环酶链（E-DNA）构建了一种用于L-组氨酸（L-His）、三聚氰胺（MA）和顺铂（CP）检测的无标记DNA酶荧光生物传感器，采用奥拉明O（AuO）染料替代硫黄素T。底物链（S-DNA）的RNA切割位点被设计为分子内茎环结构，富含GT的序列被封闭在双链茎中，从而抑制其与AuO形成稳定的G-四链体（G4cpx）。L-His...