Fluorescent detection of nisin by genetically modified Lactococcus lactis strains in milk and a colonic model: Application of whole-cell nisin biosensors.
在肠道等复杂生态系统中检测生物活性肽具有挑战性。本研究以不产乳链菌肽的乳链球菌 Lactococcus lactis NZ9000 为宿主,构建两种新型 nisin 荧光生物报告菌:分别转入 pNZ:Nis-aFP 或 pNZ:Nis-mCherry 质粒,表达厌氧荧光蛋白 evoglow-Pp1(aFP)或红色荧光蛋白 mCher...