Development of dual-color total internal reflection fluorescence biosensor for simultaneous quantitation of two small molecules and their affinity constants with antibodies.

本文开发了一种新型双色全内反射荧光生物传感器（DTB），用于同时定量两种小分子及其与抗体的亲和常数。该传感器采用单-多模光纤耦合器替代复杂共聚焦光学系统，传输两路激发光并收集双色荧光；采用光电二极管替代光电倍增管，基于光纤开关的时间分辨效应同时检测两种荧光。紧凑光学结构减少了光学分离元件和严格光路对准，提高了光传输效率和检测灵敏度。...