A Third Generation Glucose Biosensor Based on Cellobiose Dehydrogenase Immobilized on a Glassy Carbon Electrode Decorated with Electrodeposited Gold Nanoparticles: Characterization and Application in Human Saliva.
本研究实现了来自热腐生菌(Corynascus thermophilus)的纤维二糖脱氢酶突变体(CtCDH C291Y)与新型玻璃碳(GC)修饰电极之间的高效直接电子转移(DET)。该电极通过直接电沉积金纳米颗粒(AuNPs)制备,并进一步用4-氨基硫酚(4-APh)和4-巯基苯甲酸(4-MBA)混合自组装单分子层修饰,以戊二醛(...