2 条结果 关键词:人基因组DNA ×

Aggregation-resistant nanozyme containing accessible magnetite nanoparticles immobilized in monodisperse-porous silica microspheres for colorimetric assay of human genomic DNA.

Journal of colloid and interface science 2019 Öğüt E, Kip Ç, Gökçal B, Tuncel A

长链生物大分子通过桥联诱导纳米酶颗粒聚集,常导致多种纳米酶失去过氧化物酶模拟活性。以常见纳米酶磁铁矿纳米颗粒(Fe3O4 NPs)用于长链生物大分子人基因组DNA(hgDNA,14,000 kDa,含22 kb)比色检测时,由于Fe3O4 NPs在hgDNA存在下发生不可逆聚集,过氧化物酶样活性消失。本研究合成了将Fe3O4 NPs...

Electrochemical detection of the MT-ND6 gene and its enzymatic digestion: application in human genomic sample.

Analytical biochemistry 2014 Mazloum-Ardakani M, Ahmadi R, Heidari MM, Sheikh-Mohseni MA

本文报道了一种用于检测线粒体 NADH 脱氢酶 6 基因(MT-ND6)及其 BamHI 限制性内切酶酶切过程的简单电化学生物传感器。该传感器以玻璃碳电极(GCE)为基底,先修饰金纳米粒子(AuNPs),再通过巯基自组装固定 MT-ND6 基因片段探针寡核苷酸(ssDNA/AuNPs/GCE)。采用亚甲基蓝(MB)和中性红(NR)作...