Recombinant expression, characterization and application of a dihydrolipoamide dehydrogenase with diaphorase activity from Bacillus sphaericus.
二氢硫辛胺脱氢酶(diaphorase)是一类含黄素酶,具有生物转化、生物传感器设计和体外诊断等潜在应用。本文报道了来自Bacillus sphaericus PAD-91菌株的一种具有二氢硫辛胺脱氢酶活性的二氢硫辛酰胺脱氢酶(DLD)的重组表达、表征与应用。DLD基因长1413 bp,编码470个氨基酸,在大肠杆菌BL21(DE3...