Amplified electrochemiluminescence detection of DNA-binding protein based on the synergy effect of electron and energy transfer between CdS nanocrystals and gold nanoparticles.
本文首次阐明了含硫化镉纳米晶(CdS NCs)与金纳米颗粒(Au NPs)的电化学发光(ECL)体系中电子转移与能量转移的协同效应,并据此构建了一种用于灵敏检测DNA-蛋白相互作用的放大ECL生物传感器。具体而言,先在玻璃碳电极(GCE)表面修饰Au NPs,再通过Au-S键锚定硫醇修饰的探针DNA;随后使修饰电极与SiO2@CdS...