Dual-channel sensing strategy based on gold nanoparticles cooperating with carbon dots and hairpin structure for assaying RNA and DNA.
本文提出一种基于金纳米颗粒(AuNPs)、荧光碳点(CDs)和发夹结构(HS)的双通道生物传感器,用于同时检测多种核苷酸序列。无靶标时,CDs-DNA 与 AuNPs-DNA 杂交,使 CDs 靠近 AuNPs 并通过荧光共振能量转移(FRET)被猝灭;HS 与 AuNPs-DNA 杂交后发夹打开,5'端 Cy3 荧光恢复。当乳腺癌...