Modulating Chromophore Flexibility in GEVIs through Threonine-Based Molecular Switches Reveals an Influence of Membrane Curvature on Protein Activity.

许多基因编码电压指示器（GEVIs）依赖荧光蛋白（FP）域报告膜电位变化。电压传感域激活时，稳态荧光快速可逆变化，提示 FP 域发色团附近发生瞬时构象变化，表明发色团柔性可能是信号调制机制。将发色团附近苯丙氨酸替换为苏氨酸（F165T）引入荧光响应的次级成分，与发色团运动性增加一致；外部方向性极化偏移（164T/166F）可重新取向...