Kinetics and interaction studies of anti-tetraspanin antibodies and ICAM-1 with extracellular vesicle subpopulations using continuous flow quartz crystal microbalance biosensor.

本研究采用连续流石英晶体微天平（QCM）无标记研究细胞外囊泡（EV）亚群与四种亲和配体的结合动力学。所研究EV亚群包括小于50 nm的exomere和50–80 nm的exosome，并通过在线联用免疫亲和色谱-非对称流场流分级（IAC-AsFlFFF）系统从人血浆中分离获得高纯度CD9+、CD63+和CD81+亚群。亲和配体为抗四...