1 条结果 关键词:固定化大肠杆菌 ×

Preparation and Assay of Simple Light off Biosensor Based on Immobilized Bioluminescent Bacteria for General Toxicity Assays.

Methods in molecular biology (Clifton, N.J.) 2016 Gabriel GV, Viviani VR

本文介绍一种用于环境一般毒性检测的简单光关闭生物发光生物传感器。该传感器以工程化大肠杆菌 BL21(DE3) 为全细胞识别与换能元件,通过 pPro 质粒分别表达 Macrolampis sp2 萤火虫荧光素酶或 Pyrearinus termitilluminans 叩甲荧光素酶,并经 IPTG 诱导。诱导后的细菌可培养于液体中,...