A Study of the Detection of SARS-CoV-2 by the Use of Electrochemiluminescent Biosensor Based on Asymmetric Polymerase Chain Reaction Amplification Strategy.
本文报道了一种基于不对称聚合酶链式反应(asymmetric PCR)扩增策略的电化学发光(ECL)生物传感器,用于高灵敏检测严重急性呼吸综合征冠状病毒2型(SARS-CoV-2)开放阅读框1ab(ORF1ab)基因。该方法以链霉亲和素修饰磁珠偶联生物素标记互补核酸序列作为磁性捕获探针,以三(2,2′-联吡啶)钌(II)(Ru(bp...