Development of surface-engineered yeast cells displaying phytochelatin synthase and their application to cadmium biosensors by the combined use of pyrene-excimer fluorescence.

开发简单、便携、低成本、快速的有毒重金属检测与监测方法对保障人类和环境安全具有重要意义。本文报道了植物螯合肽合成酶（PC synthase, PCS）在镉（Cd）新型荧光传感平台中的应用。首先构建了表面工程化酵母细胞，在其表面展示拟南芥 PCS1（AtPCS1）并保持酶活性。该传感器基于 AtPCS1 展示酵母细胞在 Cd 浓度依赖...